Journal: Immunity
Article Title: The SPPL3-Defined Glycosphingolipid Repertoire Orchestrates HLA Class I-Mediated Immune Responses
doi: 10.1016/j.immuni.2020.11.003
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: This paper N/A Recombinant DNA pX330 Addgene Plasmid #42230 TIA-2A-blast Dr. T. Brummelkamp (NKI, the Netherlands) Blomen et al., 2015 LentiCRISPR_v2 Addgene Plasmid #52961 pL-CRISPR.EFS.GFP Addgene Plasmid #57818 pMXs-puro retroviral expression vector Cell Biolabs Cat# RTV-012 pMXs-puro-GFP This paper N/A pMXs-puro-GFP-SPPL3 This paper Voss et al., 2012 pMXs-puro-GFP-SPPL3DA This paper Voss et al., 2012 pMXs-puro-FLAG-N This paper N/A pMXs-puro-FLAG-C This paper N/A pMXs-puro-FLAG-B3GNT5 This paper IMAGE:202800754 pMXs-puro-B3GNT5-FLAG This paper IMAGE:202800754 pMXs-puro-RFP This paper N/A pMXs-puro-RFP-SPPL3 This paper Voss et al., 2012 pMXs-puro-RFP-SPPL3DA This paper Voss et al., 2012 pMXs-puro-FLAG-B4GALNT1 This paper IMAGE:202800771 pMXs-puro-FLAG-ST3GAL5 This paper IMAGE:202759803 puc2CL6IN-HLA-C*05:01 (with mutated signal peptide from HLA-A*02:01 [M4V]) Dr. A. Halenius (University Medical Center Freiburg) N/A pLZRS-HLA-A*02:01-IRES-ΔNGFR Dr. M. Griffioen (LUMC, the Netherlands) ( Griffioen et al., 2012 ; Van Bergen et al., 2010 ) pLZRS-HLA-B*40:01-IRES-ΔNGFR Dr. M. Griffioen (LUMC, the Netherlands) ( Griffioen et al., 2012 ; Van Bergen et al., 2010 ) pLZRS-HLA-C*03:03-IRES-ΔNGFR Dr. M. Griffioen (LUMC, the Netherlands) ( Griffioen et al., 2012 ; Van Bergen et al., 2010 ) Software and Algorithms Phyre2 http://www.sbg.bio.ic.ac.uk/ Kelley et al., 2015 PyMOL v2.0 http://pymol.org Schrödinger, LCC Compass Data Analysis v4.0 and v5.0 software http://www.bruker.com Bruker Daltonics FlowJo Single Cell Analysis v10 software http://www.flowjo.com FlowJo, LLC Fiji - plugin ThunderSTORM Ovesny et al., 2014 Glycoworkbench Ceroni et al., 2008 Glycomod Cooper et al., 2001 TCGA survival and expression data http://oncoLnc.org Anaya, 2016 Graphpad Prism http://www.graphpad.com N/A Other RFP-Trap_A antibody ChromoTek Cat# rta-20, RRID:AB_2631362 Acclaim PepMap100 C18 column, 100 μm × 2cm, C18 particle size 5 μm, pore size 100υ.
Techniques: Recombinant, Derivative Assay, Enzyme-linked Immunosorbent Assay, Sequencing, CRISPR, Plasmid Preparation, Expressing, Software, Single-cell Analysis
